质粒名称: | pMuLE Lenti Dest iRFP |
---|---|
出品公司: | Addgene |
目录编号: | 62176 |
存储实验室: | Ian Frew |
载体骨架基本信息 |
|
载体名称: | -- |
载体出品公司: | -- |
空载体大小: | -- |
完整质粒大小: | -- |
载体修饰: | -- |
载体类型: | 哺乳动物表达,慢病毒;MuLE网关目标向量 |
筛选标记: | iRFP |
载体抗性: | 氯霉素和氨苄霉素 |
生长温度: | 37 ℃ |
宿主菌: | DB3.1 |
拷贝数: | 高拷贝 |
插入基因信息 |
|
插入基因名称: | -- |
物种来源: | -- |
插入大小: | -- |
基因突变: | -- |
GenBank编号 | -- |
启动子: | -- |
标签/融合蛋白: | -- |
克隆方法: | -- |
5’克隆位点: | -- |
3’克隆位点: | -- |
5’测序引物 | -- |
3’测序引物 | -- |
文献引用方法 |
|
材料和方法部分: | pMuLE Lenti Dest iRFP was a gift from Ian Frew (Addgene plasmid # 62176 ; http://n2t.net/addgene:62176 ; RRID:Addgene_62176) |
参考文献部分: | A versatile modular vector system for rapid combinatorial mammalian genetics. Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, Bode-Lesniewska B, Wild PJ, Frew IJ. J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743. 10.1172/JCI79743 PubMed 25751063 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pMuLE Lenti Dest iRFP 9377 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION MuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses iRFP under the control of a PGK promoter. Compatible with MultiSite Gateway cloning. ACCESSION . VERSION . KEYWORDS pMuLE Lenti Dest iRFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9377) AUTHORS Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, Bode-Lesniewska B, Wild PJ, Frew IJ TITLE A versatile modular vector system for rapid combinatorial mammalian genetics. JOURNAL J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743. PUBMED 25751063 REFERENCE 2 (bases 1 to 9377) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9377 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 94..327 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 399..520 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(436..455) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 490..509 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 539..674 /label=SV40 ori /note="SV40 origin of replication" primer_bind 601..620 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(706..725) /label=T7 /note="T7 promoter, forward primer" promoter complement(707..725) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(735..752) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(735..751) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(744..766) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 896..1351 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(983..1002) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 1193..1214 /label=F1ori-F /note="F1 origin, forward primer" promoter 1380..1484 /gene="bla" /label=AmpR promoter CDS 1485..2345 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(1703..1722) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 2516..3104 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3005..3024 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3258..3275 /label=L4440 /note="L4440 vector, forward primer" protein_bind 3392..3413 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 3428..3458 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 3466..3482 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 3471..3493 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 3490..3506 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 3490..3506 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 3525..3545 /label=T3 /note="T3 promoter, forward primer" promoter 3527..3545 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" LTR 3801..3981 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 4025..4150 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 4643..4876 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 5060..5104 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 5365..5482 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" protein_bind 5537..5661 /gene="mutant version of attR" /label=attR1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter 5686..5716 /label=lac UV5 promoter /note="E. coli lac promoter with an ""up"" mutation" CDS 5770..6429 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind complement(5836..5855) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" CDS 6771..7076 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" primer_bind 6955..6974 /label=ccdB-fwd /note="ccdB gene, forward primer" protein_bind complement(7117..7241) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" misc_feature 7272..7860 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(7325..7345) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(7743..7754) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" promoter 7874..8373 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" primer_bind complement(7909..7932) /label=MSCV-rev /note="Murine stem cell virus, reverse primer" primer_bind 8306..8325 /label=mPGK-F /note="Mouse PGK promoter, forward primer" regulatory 8411..8420 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 8417..9367 /codon_start=1 /product="phytochrome-based near-infrared fluorescent protein, originally termed iRFP (Filonov et al., 2011)" /label=iRFP713 /note="name was changed to iRFP713 (Shcherbakova and Verkhusha, 2013)" /translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGS DNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFI GSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKIT GFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDI NYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGERLWGL IVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE" ORIGIN 1 cgcgtatgca tctcgagggc ccggtacctt taagaccaat gacttacaag gcagctgtag 61 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctagctcac tcccaacgaa 121 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg 181 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc 241 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct 301 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt 361 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata 421 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc 481 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat 541 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt 601 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg 661 cttttttgga ggcctaggct tttgcgtcga gacgtaccca attcgcccta tagtgagtcg 721 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt 781 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag 841 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcgacgcg 901 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca 961 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc 1021 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct 1081 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg 1141 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc 1201 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg 1261 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg 1321 aattttaaca aaatattaac gtttacaatt tcccaggtgg cacttttcgg ggaaatgtgc 1381 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac 1441 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt 1501 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag 1561 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg 1621 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa 1681 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc 1741 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag 1801 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa 1861 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc 1921 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg 1981 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa 2041 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa 2101 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg 2161 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag 2221 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg 2281 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt 2341 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt 2401 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac 2461 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag 2521 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg 2581 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca 2641 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga 2701 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca 2761 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc 2821 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca 2881 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa 2941 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc 3001 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc 3061 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg 3121 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat 3181 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca 3241 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca 3301 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg 3361 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac 3421 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac 3481 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact 3541 aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc 3601 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc 3661 atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg 3721 tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg 3781 cctagctcga tacaataaac gggtctctct ggttagacca gatctgagcc tgggagctct 3841 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag 3901 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt 3961 cagtgtggaa aatctctagc agtggcgccc gaacagggac ctgaaagcga aagggaaacc 4021 agagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg 4081 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg 4141 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag 4201 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga 4261 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg 4321 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt 4381 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga 4441 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct 4501 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag 4561 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga 4621 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa 4681 gcactatggg cgcagcctca atgacgctga cggtacaggc cagacaatta ttgtctggta 4741 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac 4801 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa 4861 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg 4921 tgccttggaa tgctagttgg agtaataaat ctctggaaca gattggaatc acacgacctg 4981 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga 5041 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag 5101 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat 5161 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt 5221 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga 5281 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt 5341 agtgaacgga tctcgacggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc 5401 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca 5461 aattacaaaa attcaaaatt ttattccagt gtggtggaat tgagtattcc agtgtggtgg 5521 aattctgcag atatcaacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat 5581 aaatatcaat atattaaatt agattttgca taaaaaacag actacataat actgtaaaac 5641 acaacatatc cagtcactat ggcggccgca ttaggcaccc caggctttac actttatgct 5701 tccggctcgt ataatgtgtg gattttgagt taggatccgg cgagattttc aggagctaag 5761 gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat 5821 cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt 5881 cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg 5941 gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg 6001 aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag 6061 caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta 6121 cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg 6181 tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat 6241 ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat 6301 acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtctgtgat 6361 ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc 6421 ggggcgtaaa gatctggatc cggcttacta aaagccagat aacagtatgc gtatttgcgc 6481 gctgattttt gcggtataag aatatatact gatatgtata cccgaagtat gtcaaaaaga 6541 ggtgtgctat gaagcagcgt attacagtga cagttgacag cgacagctat cagttgctca 6601 aggcatatat gatgtcaata tctccggtct ggtaagcaca accatgcaga atgaagcccg 6661 tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg 6721 gtttattgaa atgaacggct cttttgctga cgagaacagg gactggtgaa atgcagttta 6781 aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata 6841 ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag 6901 ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga 6961 tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca 7021 gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt 7081 caggctccgt tatacacagc cagtctgcag gtcgaccata gtgactggat atgttgtgtt 7141 ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata 7201 tcattttacg tttctcgttc agctttcttg tacaaagtgg ttgatatcca gcacagtggc 7261 ggccgctcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta 7321 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta 7381 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt 7441 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg 7501 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt 7561 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag 7621 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc 7681 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc 7741 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc 7801 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc 7861 ctggaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta 7921 gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca 7981 tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact 8041 cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa 8101 atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag 8161 cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag 8221 aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc 8281 gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg 8341 ctgttctcct cttcctcatc tccgggcctt tcgacctgca gcccaagctt accacactcc 8401 tgcatctgcc gccaccatgg cggaaggatc cgtcgccagg cagcctgacc tcttgacctg 8461 cgacgatgag ccgatccata tccccggtgc catccaaccg catggactgc tgctcgccct 8521 cgccgccgac atgacgatcg ttgccggcag cgacaacctt cccgaactca ccggactggc 8581 gatcggcgcc ctgatcggcc gctctgcggc cgatgtcttc gactcggaga cgcacaaccg 8641 tctgacgatc gccttggccg agcccggggc ggccgtcgga gcaccgatca ctgtcggctt 8701 cacgatgcga aaggacgcag gcttcatcgg ctcctggcat cgccatgatc agctcatctt 8761 cctcgagctc gagcctcccc agcgggacgt cgccgagccg caggcgttct tccgccgcac 8821 caacagcgcc atccgccgcc tgcaggccgc cgaaaccttg gaaagcgcct gcgccgccgc 8881 ggcgcaagag gtgcggaaga ttaccggctt cgatcgggtg atgatctatc gcttcgcctc 8941 cgacttcagc ggcgaagtga tcgcagagga tcggtgcgcc gaggtcgagt caaaactagg 9001 cctgcactat cctgcctcaa ccgtgccggc gcaggcccgt cggctctata ccatcaaccc 9061 ggtacggatc attcccgata tcaattatcg gccggtgccg gtcaccccag acctcaatcc 9121 ggtcaccggg cggccgattg atcttagctt cgccatcctg cgcagcgtct cgcccgtcca 9181 tctggaattc atgcgcaaca taggcatgca cggcacgatg tcgatctcga ttttgcgcgg 9241 cgagcgactg tggggattga tcgtttgcca tcaccgaacg ccgtactacg tcgatctcga 9301 tggccgccaa gcctgcgagc tagtcgccca ggttctggcc tggcagatcg gcgtgatgga 9361 agagtgagtc gacgcga //
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
- 上一篇:pKOdeltagalK (Hyg)
- 下一篇:pSBL_DK193